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Relic DNA is abundant in soil and obscures estimates of soil microbial diversity [Nature Microbiology]

Keywords : biogeochemistry, microbial ecology

par Frédéric Magné - publié le

Extracellular DNA from dead microorganisms can persist in soil for weeks to years. Although it is implicitly assumed that the microbial DNA recovered from soil predominantly represents intact cells, it is unclear how extracellular DNA affects molecular analyses of microbial diversity. We examined a wide range of soils using viability PCR based on the photoreactive DNA-intercalating dye propidium monoazide.(...)

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